Large-scale characterization of DNA methylation changes in human gastric carcinomas with and without metastasis.

ABSTRACT:

Purpose

Metastasis is the leading cause of death for gastric carcinoma. An epigenetic biomarker panel for predicting gastric carcinoma metastasis could have significant clinical impact on the care of patients with gastric carcinoma. The main purpose of this study is to characterize the methylation differences between gastric carcinomas with and without metastasis.

Experimental design

Genome-wide DNA methylation profiles between 4 metastatic and 4 nonmetastatic gastric carcinomas and their surgical margins (SM) were analyzed using methylated-CpG island amplification with microarray. The methylation states of 73 candidate genes were further analyzed in patients with gastric carcinoma in a discovery cohort (n=108) using denatured high performance liquid chromatography, bisulfit

SUBMITTER: Liu Z 

PROVIDER: S-EPMC4309661 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature