Evaluation of commercially available RNA amplification kits for RNA sequencing using very low input amounts of total RNA.

ABSTRACT: This article includes supplemental data. Please visit http://www.fasebj.org to obtain this information.Multiple recent publications on RNA sequencing (RNA-seq) have demonstrated the power of next-generation sequencing technologies in whole-transcriptome analysis. Vendor-specific protocols used for RNA library construction often require at least 100 ng total RNA. However, under certain conditions, much less RNA is available for library construction. In these cases, effective transcriptome profiling requires amplification of subnanogram amounts of RNA. Several commercial RNA amplification kits are available for amplification prior to library construction for next-generation sequencing, but these kits have not been comprehensively field evaluated for accuracy and performance of RNA-seq for pi

SUBMITTER: Shanker S 

PROVIDER: S-EPMC4310221 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature