Elucidation of the RamA regulon in Klebsiella pneumoniae reveals a role in LPS regulation.

ABSTRACT: Klebsiella pneumoniae is a significant human pathogen, in part due to high rates of multidrug resistance. RamA is an intrinsic regulator in K. pneumoniae established to be important for the bacterial response to antimicrobial challenge; however, little is known about its possible wider regulatory role in this organism during infection. In this work, we demonstrate that RamA is a global transcriptional regulator that significantly perturbs the transcriptional landscape of K. pneumoniae, resulting in altered microbe-drug or microbe-host response. This is largely due to the direct regulation of 68 genes associated with a myriad of cellular functions. Importantly, RamA directly binds and activates the lpxC, lpxL-2 and lpxO genes associated with lipid A biosynthesis, thus resulting in modificat

SUBMITTER: De Majumdar S 

PROVIDER: S-EPMC4310594 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature