Methylome analysis identifies a Wilms tumor epigenetic biomarker detectable in blood.

ABSTRACT:

Background

Wilms tumor is the most common pediatric renal malignancy and there is a clinical need for a molecular biomarker to assess treatment response and predict relapse. The known mutated genes in this tumor type show low mutation frequencies, whereas aberrant methylation at 11p15 is by far the most common aberration. We therefore analyzed the epigenome, rather than the genome, to identify ubiquitous tumor-specific biomarkers.

Results

Methylome analysis of matched normal kidney and Wilms tumor identifies 309 preliminary methylation variable positions which we translate into three differentially methylated regions (DMR) for use as tumor-specific biomarkers. Using two novel algorithms we show that these three DMRs are not confounded by cell type composition. We further sho

SUBMITTER: Charlton J 

PROVIDER: S-EPMC4310621 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature