Microcontroller-assisted compensation of adenosine triphosphate levels: instrument and method development.

ABSTRACT: In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2-48 μM. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme.

SUBMITTER: Hu JB 

PROVIDER: S-EPMC4311256 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature