Application of Statistically Based Experimental Designs to Optimize Cellulase Production and Identification of Gene.

ABSTRACT: A natural bacterial strain identified as Bacillus amyloliquefaciens MBAA3 using 16S rDNA partial genome sequencing has been studied for optimization of cellulase production. Statistical screening of media components for production of cellulase by B. amyloliquefaciens MBAA3 was carried out by Plackett-Burman design. Plackett-Burman design showed CMC, MgSO4 and pH as significant components influencing the cellulase production from the media components screened by Plackett-Burman fractional factorial design. The optimum concentrations of these significant parameters were determined employing the response surface central composite design, involving three factors and five levels was adopted to acquire the best medium for the production of cellulase enzyme revealed concentration of CMC (1.84 g),

SUBMITTER: Thakkar A 

PROVIDER: S-EPMC4311583 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature