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YAP regulates S-phase entry in endothelial cells.

ABSTRACT: The Hippo pathway regulates cell proliferation and apoptosis through the Yes-associated protein (YAP) transcriptional activator. YAP has a well-described role in promoting cell proliferation and survival, but the precise mechanisms and transcriptional targets that underlie these properties are still unclear and likely context-dependent. We found, using siRNA-mediated knockdown, that YAP is required for proliferation in endothelial cells but not HeLa cells. Specifically, YAP is required for S-phase entry and its absence causes cells to accumulate in G1. Microarray analysis suggests that YAP mediates this effect by regulating the transcription of genes involved in the assembly and/or firing of replication origins and homologous recombination of DNA. These findings thus provide insight into the molecular mechanisms by which YAP regulates cell cycle progression.

SUBMITTER: Shen Z

PROVIDER: S-EPMC4312014 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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YAP regulates S-phase entry in endothelial cells.

Shen Zhewei Z Stanger Ben Z BZ

PloS one 20150130 1

The Hippo pathway regulates cell proliferation and apoptosis through the Yes-associated protein (YAP) transcriptional activator. YAP has a well-described role in promoting cell proliferation and survival, but the precise mechanisms and transcriptional targets that underlie these properties are still unclear and likely context-dependent. We found, using siRNA-mediated knockdown, that YAP is required for proliferation in endothelial cells but not HeLa cells. Specifically, YAP is required for S-pha ...[more]

PMID: 25635998

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Phase separation of YAP-MAML2 differentially regulates the transcriptome.

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YAP Overexpression in Breast Cancer Cells Promotes Angiogenesis through Activating YAP Signaling in Vascular Endothelial Cells

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Phase separation of YAP-MAML2 differentially regulates the transcriptome

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TNF-α-Induced YAP/TAZ Activity Mediates Leukocyte-Endothelial Adhesion by Regulating VCAM1 Expression in Endothelial Cells.

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| S-EPMC4472080 | biostudies-other

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