Ischemic stroke activates hematopoietic bone marrow stem cells.

ABSTRACT:

Rationale

The mechanisms leading to an expanded neutrophil and monocyte supply after stroke are incompletely understood.

Objective

To test the hypothesis that transient middle cerebral artery occlusion (tMCAO) in mice leads to activation of hematopoietic bone marrow stem cells.

Methods and results

Serial in vivo bioluminescence reporter gene imaging in mice with tMCAO revealed that bone marrow cell cycling peaked 4 days after stroke (P<0.05 versus pre tMCAO). Flow cytometry and cell cycle analysis showed activation of the entire hematopoietic tree, including myeloid progenitors. The cycling fraction of the most upstream hematopoietic stem cells increased from 3.34%±0.19% to 7.32%±0.52% after tMCAO (P<0.05). In vivo microscopy corroborated proliferation of adoptively t

SUBMITTER: Courties G 

PROVIDER: S-EPMC4312511 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature