Intestinal microbiota-derived metabolomic blood plasma markers for prior radiation injury.

ABSTRACT:

Purpose

Assessing whole-body radiation injury and absorbed dose is essential for remediation efforts following accidental or deliberate exposure in medical, industrial, military, or terrorist incidents. We hypothesize that variations in specific metabolite concentrations extracted from blood plasma would correlate with whole-body radiation injury and dose.

Methods and materials

Groups of C57BL/6 mice (n=12 per group) were exposed to 0, 2, 4, 8, and 10.4 Gy of whole-body gamma radiation. At 24 hours after treatment, all animals were euthanized, and both plasma and liver biopsy samples were obtained, the latter being used to identify a distinct hepatic radiation injury response within plasma. A semiquantitative, untargeted metabolite/lipid profile was developed using gas chrom

SUBMITTER: O Broin P 

PROVIDER: S-EPMC4312583 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature