Digital gene expression analysis of gene expression differences within Brassica diploids and allopolyploids.

ABSTRACT:

Background

Brassica includes many successfully cultivated crop species of polyploid origin, either by ancestral genome triplication or by hybridization between two diploid progenitors, displaying complex repetitive sequences and transposons. The U's triangle, which consists of three diploids and three amphidiploids, is optimal for the analysis of complicated genomes after polyploidization. Next-generation sequencing enables the transcriptome profiling of polyploids on a global scale.

Results

We examined the gene expression patterns of three diploids (Brassica rapa, B. nigra, and B. oleracea) and three amphidiploids (B. napus, B. juncea, and B. carinata) via digital gene expression analysis. In total, the libraries generated between 5.7 and 6.1 million raw reads, and the clea

SUBMITTER: Jiang J 

PROVIDER: S-EPMC4312607 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature