Development and validation of a sensitive and specific sodB-based quantitative PCR assay for molecular detection of Ehrlichia species.

ABSTRACT: We developed a sensitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp. The assay's limit of detection was 5 copies/reaction, and it did not amplify nonspecific DNA. Compared with a 16S rRNA gene PCR target, the sodB target may offer an improved molecular diagnostic assay to detect Ehrlichia spp.

SUBMITTER: Qurollo BA 

PROVIDER: S-EPMC4313244 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature