Whole-genome comparison of Aspergillus fumigatus strains serially isolated from patients with aspergillosis.

ABSTRACT: The emergence of azole-resistant strains of Aspergillus fumigatus during treatment for aspergillosis occurs by a mutation selection process. Understanding how antifungal resistance mechanisms evolve in the host environment during infection is of great clinical importance and biological interest. Here, we used next-generation sequencing (NGS) to identify mutations that arose during infection by A. fumigatus strains sequentially isolated from two patients, one with invasive pulmonary aspergillosis (IPA) (five isolations) and the other with aspergilloma (three isolations). The serial isolates had identical microsatellite types, but their growth rates and conidia production levels were dissimilar. A whole-genome comparison showed that three of the five isolates from the IPA patient carried a m

SUBMITTER: Hagiwara D 

PROVIDER: S-EPMC4313286 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature