Microfluidic device with tunable post arrays and integrated electrodes for studying cellular release.

ABSTRACT: In this paper, we describe the development of a planar, pillar array device that can be used to image either side of a tunable membrane, as well as sample and detect small molecules in a cell-free region of the microchip. The pores are created by sealing two parallel PDMS microchannels (a cell channel and a collector channel) over a gold pillar array (5 or 10 μm in height), with the device being characterized and optimized for small molecule cross-over while excluding a flowing cell line (here, red blood cells, RBCs). The device was characterized in terms of the flow rate dependence of analyte cross-over and cell exclusion as well as the ability to perform amperometric detection of catechol and nitric oxide (NO) as they cross-over into the collector channel. Using catechol as the test anal

SUBMITTER: Selimovic A 

PROVIDER: S-EPMC4313528 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature