Structure and function of phosphonoacetaldehyde dehydrogenase: the missing link in phosphonoacetate formation.

ABSTRACT: Phosphonates (C-PO₃²⁻) have applications as antibiotics, herbicides, and detergents. In some environments, these molecules represent the predominant source of phosphorus, and several microbes have evolved dedicated enzymatic machineries for phosphonate degradation. For example, most common naturally occurring phosphonates can be catabolized to either phosphonoacetaldehyde or phosphonoacetate, which can then be hydrolyzed to generate inorganic phosphate and acetaldehyde or acetate, respectively. The phosphonoacetaldehyde oxidase gene (phnY) links these two hydrolytic processes and provides a previously unknown catabolic mechanism for phosphonoacetate production in the microbial metabolome. Here, we present biochemical characterization of PhnY and high-resolution crystal structures of the ap

SUBMITTER: Agarwal V 

PROVIDER: S-EPMC4313731 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature