Loss of citron kinase affects a subset of progenitor cells that alters late but not early neurogenesis in the developing rat retina.

ABSTRACT:

Purpose

To understand how loss of citron kinase (CitK) affects retinal progenitor cells (RPCs) in the developing rat retina.

Methods

We compared knockout (KO) and wild-type (WT) retinae by immunohistochemistry. The TdT-mediated dUTP terminal nick-end labeling (TUNEL) assay was performed to determine cell death. Pulse-chase experiments using 5-ethynyl-2'-deoxyuridine (EdU) were carried out to interrogate RPC behavior and in turn neurogenesis.

Results

Reverse transcription-polymerase chain reaction analysis showed that CitK was expressed at embryonic day (E)12 and was turned off at approximately postnatal day (P)4. Immunohistochemistry showed CitK being localized as puncta at the apical end of the outer neuroblastic layer (ONBL). Analyses during embryonic development sh

SUBMITTER: Karunakaran DK 

PROVIDER: S-EPMC4313794 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature