Antigen-loaded dendritic cell migration: MR imaging in a pancreatic carcinoma model.

ABSTRACT:

Purpose

To test the following hypotheses in a murine model of pancreatic cancer: (a) Vaccination with antigen-loaded iron-labeled dendritic cells reduces T2-weighted signal intensity at magnetic resonance (MR) imaging within peripheral draining lymph nodes ( LN lymph node s) and (b) such signal intensity reductions are associated with tumor size changes after dendritic cell vaccination.

Materials and methods

The institutional animal care and use committee approved this study. Panc02 cells were implanted into the flanks of 27 C57BL/6 mice bilaterally. After tumors reached 10 mm, cell viability was evaluated, and iron-labeled dendritic cell vaccines were injected into the left hind footpad. The mice were randomly separated into the following three groups (n = 9 in each): Group

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC4314117 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature