Bone morphogenetic protein 7 (BMP-7) influences tendon-bone integration in vitro.

ABSTRACT:

Introduction

Successful graft ingrowth following reconstruction of the anterior cruciate ligament is governed by complex biological processes at the tendon-bone interface. The aim of this study was to investigate in an in vitro study the effects of bone morphogenetic protein 7 (BMP-7) on tendon-bone integration.

Materials and methods

To study the biological effects of BMP-7 on the process of tendon-bone-integration, two independent in vitro models were used. The first model involved the mono- and coculture of bovine tendon specimens and primary bovine osteoblasts with and without BMP-7 exposure. The second model comprised the mono- and coculture of primary bovine osteoblasts and fibroblasts. Alkaline phosphatase (ALP), lactate dehydrogenase (LDH), lactate and osteocalcin (OC

SUBMITTER: Schwarting T 

PROVIDER: S-EPMC4314204 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature