Maternal high-fat diet and obesity compromise fetal hematopoiesis.

ABSTRACT:

Objective

Recent evidence indicates that the adult hematopoietic system is susceptible to diet-induced lineage skewing. It is not known whether the developing hematopoietic system is subject to metabolic programming via in utero high-fat diet (HFD) exposure, an established mechanism of adult disease in several organ systems. We previously reported substantial losses in offspring liver size with prenatal HFD. As the liver is the main hematopoietic organ in the fetus, we asked whether the developmental expansion of the hematopoietic stem and progenitor cell (HSPC) pool is compromised by prenatal HFD and/or maternal obesity.

Methods

We used quantitative assays, progenitor colony formation, flow cytometry, transplantation, and gene expression assays with a series of dietary mani

SUBMITTER: Kamimae-Lanning AN 

PROVIDER: S-EPMC4314531 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature