Decoupling of evolutionary changes in transcription factor binding and gene expression in mammals.

ABSTRACT: To understand the evolutionary dynamics between transcription factor (TF) binding and gene expression in mammals, we compared transcriptional output and the binding intensities for three tissue-specific TFs in livers from four closely related mouse species. For each transcription factor, TF-dependent genes and the TF binding sites most likely to influence mRNA expression were identified by comparing mRNA expression levels between wild-type and TF knockout mice. Independent evolution was observed genome-wide between the rate of change in TF binding and the rate of change in mRNA expression across taxa, with the exception of a small number of TF-dependent genes. We also found that binding intensities are preferentially conserved near genes whose expression is dependent on the TF, and the con

SUBMITTER: Wong ES 

PROVIDER: S-EPMC4315291 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature