Genes transactivated by hepatitis C virus core protein, a microarray assay.
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ABSTRACT: Aim
To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection.Methods
Reverse transcribed cDNA was subjected to microarray assay. The coding gene transactivated by HCV core protein was cloned and analyzed with bioinformatics methods.Results
The expressive vector of pcDNA3.1(-)-core was constructed and confirmed by restriction enzyme digestion and DNA sequencing and approved correct. mRNA was purified from HepG2 and HepG2 cells transfected with pcDNA3.1(-)-core, respectively. The cDNA derived was subjected to microarray assay. A new gene named HCTP4 was cloned with molecular biological method in combination with bioinformatics method.Conclusion
HCV core is a potential transactivator. Microarray is an efficient and convenient method for analysis of differentially expressed genes.
SUBMITTER: Liu M
PROVIDER: S-EPMC4315987 | biostudies-literature | 2005 Jun
REPOSITORIES: biostudies-literature
Publications
Genes transactivated by hepatitis C virus core protein, a microarray assay.
Liu Min M Zhang Shu-Lin SL Cheng Jun J Liu Yan Y Wang Lin L Shao Qing Q Zhang Jian J Lin Shu-Mei SM
World journal of gastroenterology 20050601 22
<h4>Aim</h4>To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection.<h4>Methods</h4>Reverse transcribed cDNA was subjected to microarray assay. The coding gene transactivated by HCV core protein was cloned and analyzed with bioinformatics methods.<h4>Results</h4>The expressive vector of pcDNA3.1(-)-core was constructed and confirmed by restriction enzyme digestion and DNA sequencing and approved correct. mRNA was p ...[more]
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