Genes transactivated by hepatitis C virus core protein, a microarray assay.

ABSTRACT:

Aim

To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection.

Methods

Reverse transcribed cDNA was subjected to microarray assay. The coding gene transactivated by HCV core protein was cloned and analyzed with bioinformatics methods.

Results

The expressive vector of pcDNA3.1(-)-core was constructed and confirmed by restriction enzyme digestion and DNA sequencing and approved correct. mRNA was purified from HepG2 and HepG2 cells transfected with pcDNA3.1(-)-core, respectively. The cDNA derived was subjected to microarray assay. A new gene named HCTP4 was cloned with molecular biological method in combination with bioinformatics method.

Conclusion

HCV core is a potential transactivat

SUBMITTER: Liu M 

PROVIDER: S-EPMC4315987 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature