Kinase domain insert containing receptor promotor controlled suicide gene system kills human umbilical vein endothelial cells.

ABSTRACT:

Aim

To evaluate the killing effect of double suicide gene mediated by adenovirus and regulated under kinase domain insert containing receptor (KDR) promoter on human umbilical vein endothelial cells.

Methods

By PCR technology, human KDR promoter gene, Escherichia coli (E. coli) cytosine deaminase (CD) gene and the herpes simple virus-thymidine kinase (TK) gene were cloned. Plasmid pKDR-CDglyTK was constructed with them. Then, a recombinant adenoviral plasmid pAdKDR-CDglyTK was constructed in a "two-step transformation protocol". The newly constructed plasmids were transfected to 293 packaging cells to grow adenoviruses, which were further propagated and purified. Human umbilical vein endothelial cells (HUVEC) were infected with a different multiplicity of infection (MOI) of

SUBMITTER: Huang ZH 

PROVIDER: S-EPMC4316017 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature