S-phase arrest after vincristine treatment may promote hepatitis B virus replication.

ABSTRACT:

Aim

To observe the effect of vincristine on hepatitis B virus (HBV) replication in vitro and to study its possible mechanisms.

Methods

Vincristine was added to the cultures of two cell lines stably expressing HBV. Then, the levels of hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg), and hepatitis B core antigen (HBcAg) in the supernatants or cytoplasm were examined using by enzyme-linked immunosorbent assay and Western blot. The HBV pregenome RNA (pgRNA) was detected using reverse transcription-PCR and real-time fluorescent quantitative PCR (RT-qPCR), and viral DNA was detected using Southern blot and RT-qPCR. Cell proliferation after drug treatment was detected using the BrdU incorporation test and the trypan blue exclusion assay. Cell cycle and cell apopt

SUBMITTER: Xu L 

PROVIDER: S-EPMC4316092 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature