Ezh2 mediated H3K27me3 activity facilitates somatic transition during human pluripotent reprogramming.

ABSTRACT: Factor induced reprogramming of fibroblasts is an orchestrated but inefficient process. At the epigenetic level, it results in drastic chromatin changes to erase the existing somatic "memory" and to establish the pluripotent state. Accordingly, alterations of chromatin regulators including Ezh2 influence iPSC generation. While the role of individual transcription factors in resetting the chromatin landscape during iPSC generation is increasingly evident, their engagement with chromatin modulators remains to be elucidated. In the current study, we demonstrate that histone methyl transferase activity of Ezh2 is required for mesenchymal to epithelial transition (MET) during human iPSC generation. We show that the H3K27me3 activity favors induction of pluripotency by transcriptionally targetin

SUBMITTER: Rao RA 

PROVIDER: S-EPMC4316165 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature