Oocyte spindle proteomics analysis leading to rescue of chromosome congression defects in cloned embryos.

ABSTRACT: Embryos produced by somatic cell nuclear transfer (SCNT) display low term developmental potential. This is associated with deficiencies in spindle composition prior to activation and at early mitotic divisions, including failure to assemble certain proteins on the spindle. The protein-deficient spindles are accompanied by chromosome congression defects prior to activation and during the first mitotic divisions of the embryo. The molecular basis for these deficiencies and how they might be avoided are unknown. Proteomic analyses of spindles isolated from normal metaphase II (MII) stage oocytes and SCNT constructs, along with a systematic immunofluorescent survey of known spindle-associated proteins were undertaken. This was the first proteomics study of mammalian oocyte spindles. The study

SUBMITTER: Han Z 

PROVIDER: S-EPMC4316211 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature