Comparison of internal transcribed spacers and intergenic spacer regions of five common Iranian sheep bursate nematodes.

ABSTRACT:

Background

Accurate identification of sheep nematodes is a critical point in epidemiological studies and monitoring of drug resistance in flocks. However, due to a close morphological similarity between the eggs and larval stages of many of these nematodes, such identification is not a trivial task. There are a number of studies showing that molecular targets in ribosomal DNA (Internal transcribed spacer 1, 2 and Intergenic spacer) are suitable for accurate identification of sheep bursate nematodes. The objective of present study was to compare the ITS1, ITS2 and IGS regions of Iranian common bursate nematodes in order to choose best target for specific identification methods.

Methods

The first and second internal transcribed spacers (ITS1and ITS2) and intergenic spacer (IGS

SUBMITTER: Nabavi R 

PROVIDER: S-EPMC4316566 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature