Expression levels and genetic polymorphisms of interleukin-2 and interleukin-10 as biomarkers of Graves' disease.

ABSTRACT: The aim of the present study was to determine whether the expression levels of interleukin (IL)-2 and IL-10 may be used as biological markers in Graves' disease (GD) patients. A total of 256 individuals, including 118 GD patients and 138 healthy individuals, were enrolled into the study. Blood samples were collected from each patient and healthy individual, which were then subjected to enzyme-linked immunosorbent assay (ELISA). Total RNA and total proteins were determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis, respectively. In addition, restriction fragment length polymorphism (RFLP) analysis was performed to detect the presence of genetic polymorphisms. The ELISA results indicated that the IL-2 and IL-10 serum levels in the

SUBMITTER: Liang C 

PROVIDER: S-EPMC4316959 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature