Functional circadian clock genes are essential for the overwintering diapause of the Northern house mosquito, Culex pipiens.

ABSTRACT: The short day lengths of late summer are used to program the overwintering adult diapause (dormancy) of the Northern house mosquito, Culex pipiens. Here, we investigated the role of clock genes in initiating this diapause and asked whether the circadian cycling of clock gene expression persists during diapause. We provide evidence that the major circadian clock genes continue to cycle throughout diapause and after diapause has been terminated. RNA interference (RNAi) was used to knock down the core circadian clock genes and to then assess the impact of the various clock genes on the ability of females to enter diapause. RNAi directed against negative circadian regulators (period, timeless and cryptochrome2) caused females that were reared under diapause-inducing, short day conditions to av

SUBMITTER: Meuti ME 

PROVIDER: S-EPMC4317241 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature