Ion mobility mass spectrometry analysis of isomeric disaccharide precursor, product and cluster ions.

ABSTRACT:

Rationale

Carbohydrates are highly variable in structure owing to differences in their anomeric configurations, monomer stereochemistry, inter-residue linkage positions and general branching features. The separation of carbohydrate isomers poses a great challenge for current analytical techniques.

Methods

The isomeric heterogeneity of disaccharide ions and monosaccharide-glycolaldehyde product ions was evaluated using electrospray traveling wave ion mobility mass spectrometry (Synapt G2 high-definition mass spectrometer) in both positive and negative ion modes.

Results

The separation of isomeric disaccharide ions was observed but not fully achieved based on their mobility profiles. The mobilities of isomeric product ions, the monosaccharide-glycolaldehydes, derived fr

SUBMITTER: Li H 

PROVIDER: S-EPMC4317727 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature