Identification of anti-CD98 antibody mimotopes for inducing antibodies with antitumor activity by mimotope immunization.

ABSTRACT: A mimotope is an antibody-epitope-mimicking peptide retrieved from a phage display random peptide library. Immunization with antitumor antibody-derived mimotopes is promising for inducing antitumor immunity in hosts. In this study, we isolated linear and constrained mimotopes from HBJ127, a tumor-suppressing anti-CD98 heavy chain mAb, and determined their abilities for induction of antitumor activity equal to that of the parent antibody. We detected elevated levels of antipeptide responses, but failed to detect reactivity against native CD98-expressing HeLa cells in sera of immunized mice. Phage display panning and selection of mimotope-immunized mouse spleen-derived antibody Fab library showed that HeLa cell-reactive Fabs were successfully retrieved from the library. This finding indicate

SUBMITTER: Saito M 

PROVIDER: S-EPMC4317797 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature