Identification of novel targets for antiangiogenic therapy by comparing the gene expressions of tumor and normal endothelial cells.

ABSTRACT: Targeting tumor angiogenesis is an established strategy for cancer therapy. Because angiogenesis is not limited to pathological conditions such as cancer, molecular markers that can distinguish between physiological and pathological angiogenesis are required to develop more effective and safer approaches for cancer treatment. To identify such molecules, we determined the gene expression profiles of murine tumor endothelial cells (mTEC) and murine normal endothelial cells using DNA microarray analysis followed by quantitative reverse transcription-polymerase chain reaction analysis. We identified 131 genes that were differentially upregulated in mTEC. Functional analysis using siRNA-mediated gene silencing revealed five novel tumor endothelial cell markers that were involved in the prolifer

SUBMITTER: Otsubo T 

PROVIDER: S-EPMC4317838 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature