Significant biological role of sp1 transactivation in multiple myeloma.

ABSTRACT:

Purpose

The transcription factor specificity protein 1 (Sp1) controls number of cellular processes by regulating the expression of critical cell cycle, differentiation, and apoptosis-related genes containing proximal GC/GT-rich promoter elements. We here provide experimental and clinical evidence that Sp1 plays an important regulatory role in multiple myeloma (MM) cell growth and survival.

Experimental design

We have investigated the functional Sp1 activity in MM cells using a plasmid with Firefly luciferase reporter gene driven by Sp1-responsive promoter. We have also used both siRNA- and short hairpin RNA-mediated Sp1 knockdown to investigate the growth and survival effects of Sp1 on MM cells and further investigated the anti-MM activity of terameprocol (TMP), a small mole

SUBMITTER: Fulciniti M 

PROVIDER: S-EPMC4318245 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature