Performance of the fourth-generation Bio-Rad GS HIV Combo Ag/Ab enzyme immunoassay for diagnosis of HIV infection in Southern Africa.

ABSTRACT:

Background

Fourth-generation HIV assays detect both antigen and antibody, facilitating detection of acute/early HIV infection. The Bio-Rad GS HIV Combo Ag/Ab assay (Bio-Rad Combo) is an enzyme immunoassay that simultaneously detects HIV p24 antigen and antibodies to HIV-1 and HIV-2 in serum or plasma.

Objective

To evaluate the performance of the Bio-Rad Combo assay for detection of HIV infection in adults from Southern Africa.

Study design

Samples were obtained from adults in Soweto and Vulindlela, South Africa and Dar es Salaam, Tanzania (300 HIV-positive samples; 300 HIV-negative samples; 12 samples from individuals previously classified as having acute/early HIV infection). The samples were tested with the Bio-Rad Combo assay. Additional testing was performed to ch

SUBMITTER: Piwowar-Manning E 

PROVIDER: S-EPMC4319362 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature