Developmental defects associated with glucosamine auxotrophy in Saccharomyces cerevisiae.

ABSTRACT: Saccharomyces cerevisiae mutants, unable to make D-glucosamine owing to a defect in the enzyme 2-amino-2-deoxy-D-glucose-6-phosphate ketol-isomerase (amino-transferring) (EC 5.3.1.19), show aberrations both in sporulation and in vegetative growth. They grow normally on a medium of yeast extract, peptone, and dextrose (YEPD) containing D-glucosamine (1 mg/ml), and such cells accumulate 4 to 5 times the amount of D-glucosamine present in wild-type cells cultured on YEPD alone. When such mutant cells are shifted to YEPD alone, they continue to increase in cell mass for about 10 hr (three to four cell cycles) and produce strings of beads in which the cells fail to separate. Although each of the "cells" contains a nucleus, electron micrographs of thin sections reveal that septation is defective

SUBMITTER: Ballou CE 

PROVIDER: S-EPMC431939 | biostudies-literature | 1977 Oct

REPOSITORIES: biostudies-literature