Identifying a kinase network regulating FGF14:Nav1.6 complex assembly using split-luciferase complementation.

ABSTRACT: Kinases play fundamental roles in the brain. Through complex signaling pathways, kinases regulate the strength of protein:protein interactions (PPI) influencing cell cycle, signal transduction, and electrical activity of neurons. Changes induced by kinases on neuronal excitability, synaptic plasticity and brain connectivity are linked to complex brain disorders, but the molecular mechanisms underlying these cellular events remain for the most part elusive. To further our understanding of brain disease, new methods for rapidly surveying kinase pathways in the cellular context are needed. The bioluminescence-based luciferase complementation assay (LCA) is a powerful, versatile toolkit for the exploration of PPI. LCA relies on the complementation of two firefly luciferase protein fragments th

SUBMITTER: Hsu WC 

PROVIDER: S-EPMC4319734 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature