Virotherapy targeting cyclin E overexpression in tumors with adenovirus-enhanced cancer-selective promoter.

ABSTRACT: Oncolytic virotherapy can selectively destroy cancer cells and is a potential approach in cancer treatment. A strategy to increase tumor-specific selectivity is to control the expression of a key regulatory viral gene with a tumor-specific promoter. We have previously found that cyclin E expression is augmented in cancer cells after adenovirus (Ad) infection. Thus, the cyclin E promoter that is further activated by Ad in cancer cells may have unique properties for enhancing oncolytic viral replication. We have shown that high levels of viral E1a gene expression are achieved in cancer cells infected with Ad-cycE, in which the endogenous Ad E1a promoter was replaced with the cyclin E promoter. Ad-cycE shows markedly selective oncolytic efficacy in vitro and destroys various types of cancer c

SUBMITTER: Cheng PH 

PROVIDER: S-EPMC4320008 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature