Direct observation of cell cycle progression in living mouse embryonic stem cells on an extracellular matrix of E-cadherin.

ABSTRACT: Self-renewal and differentiation of embryonic stem cells are tightly coordinated with cell-cycle progression and reconstructions. However, technical approach to directly visualize single embryonic stem cells still remains challenging. Here we combined two independent systems by using artificially constructed extracellular matrix that maintains embryonic stem cells in single level with cell cycle visualization reporters to directly observe cell cycle progression. Using Fucci (fluorescent ubiquitination-based cell cycle indicator) technology and computer-assisted fluorescence microscopy we were able to visualize cell cycle progression of mouse embryonic stem cells prepared from Fucci2 knock-in mice (mES/Fucci2). Imaged mES/Fucci2 cells were plated on coverslips coated with recombinant E-cadh

SUBMITTER: Jovic D 

PROVIDER: S-EPMC4320234 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature