Investigation of protein secretion and secretion stress in Ashbya gossypii.

ABSTRACT:

Background

Ashbya gossypii is a filamentous Saccharomycete used for the industrial production of riboflavin that has been recently explored as a host system for recombinant protein production. To gain insight into the protein secretory pathway of this biotechnologically relevant fungus, we undertook genome-wide analyses to explore its secretome and its transcriptional responses to protein secretion stress.

Results

A computational pipeline was used to predict the inventory of proteins putatively secreted by A. gossypii via the general secretory pathway. The proteins actually secreted by this fungus into the supernatants of submerged cultures in minimal and rich medium were mapped by two-dimensional gel electrophoresis, revealing that most of the A. gossypii secreted proteins

SUBMITTER: Aguiar TQ 

PROVIDER: S-EPMC4320514 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature