Identification of fragments from Autographa californica polyhedrin protein essential for self-aggregation and exogenous protein incorporation.

ABSTRACT:

Background

Baculoviruses are widely used for the production of recombinant proteins, biopesticides and as gene delivery systems. One of the viral forms called polyhedra has been recently exploited as a scaffold system to incorporate or encapsulate foreign proteins or peptide fragments. However, an efficient strategy for foreign protein incorporation has not been thoroughly studied.

Results

Based on the crystal structure of polyhedrin, we conducted an in silico analysis of the baculovirus Autographa californica nucleopolyhedrovirus (AcMNPV) polyhedrin protein to select the minimum fragments of polyhedrin that could be incorporated into polyhedra. Using confocal and transmission electron microscopy we analyzed the expression and cellular localization of the different polyhedri

SUBMITTER: Sampieri A 

PROVIDER: S-EPMC4320575 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature