Assembling the protein architecture of the budding yeast kinetochore-microtubule attachment using FRET.

ABSTRACT:

Background

The kinetochore is a multiprotein machine that couples chromosome movement to microtubule (MT) polymerization and depolymerization. It uses numerous copies of at least three MT-binding proteins to generate bidirectional movement. The nanoscale organization of these proteins within the kinetochore plays an important role in shaping the mechanisms that drive persistent, bidirectional movement of the kinetochore.

Results

We used fluorescence resonance energy transfer (FRET) between genetically encoded fluorescent proteins fused to kinetochore subunits to reconstruct the nanoscale organization of the budding yeast kinetochore. We performed >60 FRET and high-resolution colocalization measurements involving the essential MT-binding kinetochore components: Ndc80, Dam1, S

SUBMITTER: Aravamudhan P 

PROVIDER: S-EPMC4320969 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature