Membrane potential depolarization causes alterations in neuron arrangement and connectivity in cocultures.

ABSTRACT:

Background

The disruption of neuron arrangement is associated with several pathologies. In contrast to action potentials, the role of resting potential (Vmem) in regulating connectivity remains unknown.

Methods

Neuron assemblies were quantified when their Vmem was depolarized using ivermectin (Ivm), a drug that opens chloride channels, for 24 h in cocultures with astrocytes. Cell aggregation was analyzed using automated cluster analysis methods. Neural connectivity was quantified based on the identification of isolated somas in phase-contrast images using image processing. Vmem was measured using voltage-sensitive dyes and whole-cell patch clamping. Immunocytochemistry and Western blotting were used to detect changes in the distribution and production of the proteins.

Res

SUBMITTER: Ozkucur N 

PROVIDER: S-EPMC4321392 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature