Dihydroxyacid dehydratase is important for gametophyte development and disruption causes increased susceptibility to salinity stress in Arabidopsis.

ABSTRACT: Dihydroxyacid dehydratase (DHAD) catalyses a key step in the branched-chain amino acid (BCAA) biosynthetic pathway that exists in numerous organisms, including bacteria, fungi, and plants, but not humans. In Arabidopsis thaliana, DHAD is encoded by a single gene (AT3G23940), but its biological function in controlling plant development remains uncharacterized. In this study, we showed that DHAD is highly expressed in most vegetative and reproductive tissues. It is an essential gene, and complete disruption caused partial sterility in both male and female gametophyte phases. In addition, reduced expression of DHAD in knockdown mutants resulted in a reduction in the accumulation of all three BCAAs in roots and, as a consequence, led to a shorter root phenotype, which could be restored by an e

SUBMITTER: Zhang C 

PROVIDER: S-EPMC4321549 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature