Replication of hepatitis B virus in primary duck hepatocytes transfected with linear viral DNA.

ABSTRACT:

Aim

To explore the expression and replication of hepatitis B virus (HBV) DNA in primary duck hepatocytes (PDHs).

Methods

Complete HBV genome was transfected into PDHs by electroporation (transfected group, 1.19 x 10(12) copies of linear HBV DNA/1 x 10(7) PDHs). After 1-5 d of transfection, HBsAg and HBeAg in the supernatant and lysate of PDHs were measured with the IMX System. Meanwhile, replicative intermediates of HBV DNA were analyzed by Southern blotting and Dot blotting. PDHs electroporated were used as control group.

Results

HBsAg in the hepatocyte lysates of transfected group was 15.24 (1 d), 14.55 (3 d) and 5.13 (5 d; P/N values, positive > or =2.1) respectively. HBeAg was negative (<2.1). Both HBsAg and HBeAg were negative in the supernatant of transfected gr

SUBMITTER: Yao YQ 

PROVIDER: S-EPMC4321921 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature