Gene expression profiles in peripheral blood mononuclear cells of SARS patients.

ABSTRACT:

Aim

To investigate the role of inflammatory and anti-viral genes in the pathogenesis of SARS.

Methods

cDNA microarrays were used to screen the gene expression profiles of peripheral blood mononuclear cells (PBMCs) in two SARS patients (one in the acute severe phase and the other in the convalescent phase) and a healthy donor. In addition, real-time qualitative PCR was also performed to verify the reproducibility of the microarray results. The data were further analyzed.

Results

Many inflammatory and anti-viral genes were differentially expressed in SARS patients. Compared to the healthy control or the convalescent case, plenty of pro-inflammatory cytokines such as IL-1, TNF-alpha, IL-8, and MAPK signaling pathway were significantly upregulated in the acute severe case

SUBMITTER: Yu SY 

PROVIDER: S-EPMC4321926 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature