Computational identification and binding analysis of orphan human cytochrome P450 4X1 enzyme with substrates.

ABSTRACT:

Background

Cytochrome P450s (CYPs) are important heme-containing proteins, well known for their monooxygenase reaction. The human cytochrome P450 4X1 (CYP4X1) is categorized as "orphan" CYP because of its unknown function. In recent studies it is found that this enzyme is expressed in neurovascular functions of the brain. Also, various studies have found the expression and activity of orphan human cytochrome P450 4X1 in cancer. It is found to be a potential drug target for cancer therapy. However, three-dimensional structure, the active site topology and substrate specificity of CYP4X1 remain unclear.

Methods

In the present study, the three-dimensional structure of orphan human cytochrome P450 4X1 was generated by homology modeling using Modeller 9v8. The generated structure

SUBMITTER: Kumar S 

PROVIDER: S-EPMC4322450 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature