Circulation of the low pathogenic avian influenza subtype H5N2 virus in ducks at a live bird market in Ibadan, Nigeria.

ABSTRACT:

Background

To monitor carrier hosts of avian influenza in Nigeria, we randomly collected cloaca swab specimens from 155 ducks at a live bird market (LBM) in Ibadan, southwest Nigeria, between July 2011 and July 2012.

Methods

The samples were analyzed by real-time reverse transcription-polymerase chain reaction (RT-PCR) and virus isolation was carried out in embryonated chicken eggs. Partial sequencing of the antigenic cleavage site of the haemagglutinin (HA) gene was performed, multiple sequence alignment was carried out using ClustalW, and a phylogenetic tree was constructed using the neighbor joining method.

Results

Twenty (13%) of the 155 samples were positive for avian influenza subtype H5N2 by real-time RT-PCR and three isolates were obtained from embryonated chi

SUBMITTER: Coker T 

PROVIDER: S-EPMC4322551 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature