Rhipicephalus microplus serine protease inhibitor family: annotation, expression and functional characterisation assessment.

ABSTRACT:

Background

Rhipicephalus (Boophilus) microplus evades the host's haemostatic system through a complex protein array secreted into tick saliva. Serine protease inhibitors (serpins) conform an important component of saliva which are represented by a large protease inhibitor family in Ixodidae. These secreted and non-secreted inhibitors modulate diverse and essential proteases involved in different physiological processes.

Methods

The identification of R. microplus serpin sequences was performed through a web-based bioinformatics environment called Yabi. The database search was conducted on BmiGi V1, BmiGi V2.1, five SSH libraries, Australian tick transcriptome libraries and RmiTR V1 using bioinformatics methods. Semi quantitative PCR was carried out using different adult tissu

SUBMITTER: Rodriguez-Valle M 

PROVIDER: S-EPMC4322644 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature