Integrated next-generation sequencing and avatar mouse models for personalized cancer treatment.

ABSTRACT:

Background

Current technology permits an unbiased massive analysis of somatic genetic alterations from tumor DNA as well as the generation of individualized mouse xenografts (Avatar models). This work aimed to evaluate our experience integrating these two strategies to personalize the treatment of patients with cancer.

Methods

We performed whole-exome sequencing analysis of 25 patients with advanced solid tumors to identify putatively actionable tumor-specific genomic alterations. Avatar models were used as an in vivo platform to test proposed treatment strategies.

Results

Successful exome sequencing analyses have been obtained for 23 patients. Tumor-specific mutations and copy-number variations were identified. All samples profiled contained relevant genomic alterati

SUBMITTER: Garralda E 

PROVIDER: S-EPMC4322867 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature