E-selectin ligand-1 controls circulating prostate cancer cell rolling/adhesion and metastasis.

ABSTRACT: Circulating prostate cancer (PCa) cells preferentially roll and adhere on bone marrow vascular endothelial cells, where abundant E-selectin and stromal cell-derived factor 1 (SDF-1) are expressed, subsequently initiating a cascade of activation events that eventually lead to the development of metastases. To elucidate the roles of circulating PCa cells' rolling and adhesion behaviors in cancer metastases, we applied a dynamic cylindrical flow-based microchannel device that is coated with E-selectin and SDF-1, mimicking capillary endothelium. Using this device we captured a small fraction of rolling PCa cells. These rolling cells display higher static adhesion ability, more aggressive cancer phenotypes and stem-like properties. Importantly, mice received rolling PCa cells, but not floating

SUBMITTER: Yasmin-Karim S 

PROVIDER: S-EPMC4322988 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature