Fast track antibody V-gene rescue, recombinant expression in plants and characterization of a PfMSP4-specific antibody.

ABSTRACT:

Background

Monoclonal antibodies (mAbs) are essential tools in biological research, diagnosis and therapy, and are conventionally produced in murine hybridoma cell lines. Professional applications of mAbs depend on the steady supply of material. Because hybridoma cultures can stop producing the antibody or even die, preservation of the unique epitope specificity of mAbs by rescue of the sequences encoding the antibody variable domains (V regions) is important. The availability of these sequences enables not only the recombinant expression of the original antibody for further applications, but opens the road for antibody engineering towards innovative diagnostic or therapeutic applications. A time- and cost-efficient production system enabling the detailed analysis of the antibodies

SUBMITTER: Kapelski S 

PROVIDER: S-EPMC4323031 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature