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Laccase engineering by rational and evolutionary design.

ABSTRACT: Laccases are considered as green catalysts of great biotechnological potential. This has attracted a great interest in designing laccases a la carte with enhanced stabilities or activities tailored to specific conditions for different fields of application. Over 20 years, numerous efforts have been taken to engineer these multicopper oxidases and to understand their reaction mechanisms by site-directed mutagenesis, and more recently, using computational calculations and directed evolution tools. In this work, we review the most relevant contributions made in the field of laccase engineering, from the comprehensive study of their structure-function relationships to the tailoring of outstanding biocatalysts.

SUBMITTER: Pardo I

PROVIDER: S-EPMC4323517 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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Laccase engineering by rational and evolutionary design.

Pardo Isabel I Camarero Susana S

Cellular and molecular life sciences : CMLS 20150114 5

Laccases are considered as green catalysts of great biotechnological potential. This has attracted a great interest in designing laccases a la carte with enhanced stabilities or activities tailored to specific conditions for different fields of application. Over 20 years, numerous efforts have been taken to engineer these multicopper oxidases and to understand their reaction mechanisms by site-directed mutagenesis, and more recently, using computational calculations and directed evolution tools. ...[more]

PMID: 25586560

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